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Since the 16S profiles within the gut look pretty different for intracellular and extracellular DNA [33], qPCR is predicted to reflect the NGS-focused Local community composition both quantitatively and qualitatively extra intently than flow cytometry.

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Melting curves and non-template controls had been used to evaluate run trustworthiness. There was no detectable amplification arising from non-template controls in almost any on the assays. The amplification efficiencies of all qPCR assays ranged from 91% to 98%.

The use of relative abundance from upcoming generation sequencing (NGS) information can cause misinterpretations of microbial community constructions as resulting from compositionality, the relative abundances with the taxa being mutually dependent. Which means that a boost of 1 taxon inevitably contributes to the concurrent lower of another(s). Considering that the alterations of parts are mutually dependent, higher Wrong discovery charges occur when compositional info are analyzed applying common statistical solutions [1].

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